Table 1.
Antibodies list used for flow cytometry, immunohistochemistry and biochemistry.
| Technique | Cat. N° | Antibody | Fluotochrome | Supplier | Clone | Dilution | Laser | Filter | Host |
|---|---|---|---|---|---|---|---|---|---|
| Flow Cytometry | 740725 | CD45 | BV711 | BDPharmingen | OX-1 | 1 in 200 | Violet 355 nm | 710/20 | mouse |
| 201807 | CD11 b/c | PE | BioLegend | OX-42 | 1 in 200 | Yellow-Green 561 nm | 596/15 | mouse | |
| 743056 | RP-1 | BV650 | BDPharmingen | RP-1 | 1 in 200 | Violet 355 nm | 677/20 | mouse | |
| 77474 | Zombie | UV | BioLegend | - | 1 in 1000 | Ultraviolet 350 nm | 450/50 | mouse | |
| 202816 | CD43 | PE/Cy7 | BioLegend | W3/13 | 1 in 200 | Yellow-Green 561 nm | 780/60 | mouse | |
| 550270 | CD32 | - | BDPharmingen | D34-485 | 1 in 200 | - | - | mouse | |
| Western Blot and IHC | AG-20B-0042 | anti-Caspase-1 | - | AdipoGen | p20 | 1 in 200 | - | - | mouse |
| ab5103 | anti-Histone H3 | - | abcam | - | 1 in 500 | - | - | rabbit | |
| 66177-1-lg | anti-MPO | - | ProteinTech | 4C11F6 | 1 in 500 | - | - | mouse | |
| ab214185 | anti-NLRP-3 | - | abcam | - | 1 in 500 | - | - | rabbit | |
| A11008 | Goat anti-rabbit Alexa Fluor 488 | - | Invitrogen | - | 1 in 400 | - | - | rabbit | |
| A11005 | Goat anti-mouse Alexa Fluor 594 | - | Invitrogen | - | 1 in 400 | - | - | mouse | |
| 35518 | Goat anti-mouse DyLight 680 | - | Invitrogen | - | 1 in 2000 | - | - | mouse | |
| 926-32211 | Goat anti-rabbit IRDye 800 | - | Licor | - | 1 in 2000 | - | - | rabbit | |
| IHC | 17-9668-82 | anti-Mo Ly-6G | APC | Invitrogen | 1AB-Ly6g | 1 in 500 | - | - | mouse |
| I32450 | Isolectin GS-IB4 | - | Invitrogen | - | 1 in 200 | - | - | rabbit | |
| ab5076 | anti-IBA-1 | - | Abcam | - | 1 in 500 | - | - | goat | |
| MAB377 | NeuN | - | Millipore | A60 | 1 in 100 | - | - | mouse |