Biofilm formation by S. marcescens and its mutants. Wild-type S. marcescens 274 and its serrawettin mutants (SMu1a, SMu4e, SMu13a, and SMu4853b [9]) were analyzed for biofilm formation in the absence and presence of surfactin as described in the legend to Fig. 3. The OD630 of all the cultures was ≈0.15 (not shown). Open bars, no surfactin; shaded bars, 100 μg of surfactin.