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. 2023 Feb 14;16(2):289. doi: 10.3390/ph16020289

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Viability of human Wharton’s jelly mesenchymal stem cells (hWJ-MSCs) after a 24 h-Cytochalasin B (CB) treatment. (a) Uncleaved (total) caspase 3 expression was investigated in untreated (CTR) or in treated hWJ-MSCs with CB 1 μM or dimethyl sulfoxide (DMSO) 0.05% (CB vehicle) for 24 h or 72 h. (b) hWJ-MSC viability was assessed using the Annexin V-7AAD staining assay. hWJ-MSCs were treated for 24 h without (CTR) or with CB (1 and 3 μM). Quadrants (Q) represent the percentage of necrotic cells (Q1), late apoptotic cells (Q2), early apoptotic cells (Q3) and living cells (Q4).