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. 2023 Feb 14;16(2):289. doi: 10.3390/ph16020289

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Immunofluorescence of human Wharton’s jelly mesenchymal stem cell (hWJ-MSC) cytoskeletal markers after 24 h of Cytochalasin B (CB) treatment. hWJ-MSCs were immunostained with Phalloidin (grey signal, specific for F-Actin), anti-vimentin (green signal), or anti-vinculin (red signal) after 24 h of dimethyl sulfoxide (DMSO) 0.05% (CB vehicle) or CB (0.1, 1, or 3 μM) exposure. Untreated cells were used as CTR. NucBlue^® Fixed Cell ReadyProbes^® Reagent (DAPI) was used to counter-stain nuclei (blue signal). Images were acquired using a Nikon inverted microscope Eclipse Ti2-E and a digital sight camera DS-Qi2, through the imaging software NIS-Elements. Scale bars: 50 μm.