Table 2.

Intra-day assays (n = 5) performed with dried blood spots (DBSs) obtained from Fabry patients as quality controls (pQCs).

				Lyso-Gb3 Analogues
		Lyso-Gb3		−28 Da		−2 Da		+16 Da		+18 Da		+34 Da
		pLQC	pHQC	pLQC	pHQC	pLQC	pHQC	pLQC	pHQC	pLQC	pHQC	pLQC	pHQC
		nM	nM	nM	nM	nM	nM	nM	nM	nM	nM	nM	nM
(A) Whatman- GE 903	Replicate 1	47.93	126.95	2.24	7.34	9.27	27.09	3.33	11.68	2.73	9.89	4.34	16.67
	Replicate 2	45.75	120.97	2.38	7.10	8.52	25.63	3.81	11.43	2.42	10.11	3.99	15.84
	Replicate 3	54.67	122.34	2.99	7.38	10.62	27.25	4.20	11.74	3.12	10.03	4.90	16.19
	Replicate 4	49.73	114.15	2.33	6.83	9.16	23.91	3.65	10.41	2.64	9.49	4.49	15.04
	Replicate 5	51.98	114.80	2.80	6.47	9.93	23.29	3.85	9.81	2.78	9.59	4.79	16.02
	Mean	50.01	119.84	2.55	7.02	9.50	25.43	3.77	11.01	2.74	9.82	4.50	15.95
	SD	3.47	5.38	0.33	0.38	0.80	1.80	0.32	0.86	0.25	0.27	0.36	0.60
	RSD%	6.9	4.5	12.8	5.4	8.4	7.1	8.4	7.8	9.3	2.8	8.0	3.7
(B) Capitainer ® B	Replicate 1	67.70	173.59	4.54	11.04	15.80	40.05	6.87	18.24	5.31	16.04	8.66	24.76
	Replicate 2	70.01	189.64	4.36	11.41	14.74	40.74	6.15	18.04	5.96	16.95	8.70	25.05
	Replicate 3	77.03	184.55	4.37	11.79	15.47	42.14	6.29	18.50	6.85	16.17	9.57	25.77
	Replicate 4	73.57	190.14	4.13	11.40	14.95	42.13	6.17	18.81	5.85	19.35	9.34	28.32
	Replicate 5	75.43	185.82	3.99	11.71	14.98	42.51	6.30	18.87	6.52	19.47	9.26	29.27
	Mean	72.75	184.75	4.28	11.47	15.19	41.51	6.36	18.49	6.10	17.59	9.10	26.63
	SD	3.85	6.68	0.22	0.30	0.43	1.06	0.29	0.36	0.60	1.69	0.41	2.03
	RSD%	5.3	3.6	5.1	2.6	2.9	2.6	4.6	1.9	9.9	9.6	4.5	7.6

SD = standard deviation; RSD = relative standard deviation; pLQC = low quality control prepared with blood from a Fabry patient; pHQC = high quality control prepared with blood from a Fabry patient.