. 2023 Feb 6;24(4):3223. doi: 10.3390/ijms24043223

Table 9.

Normal reference values (99th percentile) established for each analyte under study following the analysis of DBSs from 12 healthy reference controls. Mean, minimum, maximum, and median values are shown, along with the sensitivity and specificity of the biomarkers.

Biomarker	Blood Type	Collection Method	Mean	Min	Max	Median	99th Percentile	Sensitivity (%)	Specificity (%)
Lyso-Gb₃ (nM)	Venous	Whatman 903	3.96	2.25	6.25	3.86	6.14	100	92
	Venous	Capitainer^®B	4.60	3.16	8.43	4.18	8.19	85	92
	Capillary	Whatman 903	4.26	2.74	6.71	4.20	6.61	95	92
	Capillary	Capitainer^®B	4.15	2.95	5.93	4.19	5.92	100	100
	Plasma	EDTA K2	0.74	0.33	1.09	0.78	1.08	100	92
Lyso-Gb₃ −28 Da (nM)	Venous	Whatman 903	0.25	nd	0.53	0.26	0.51	55	92
	Venous	Capitainer^®B	nd	nd	0.42	nd	0.40	85	92
	Capillary	Whatman 903	0.28	nd	0.58	0.27	0.56	60	92
	Capillary	Capitainer^®B	nd	nd	0.24	nd	0.24	100	92
	Plasma	EDTA K2	nd	nd	nd	nd	nd	100	92
Lyso-Gb₃ −2 Da (nM)	Venous	Whatman 903	0.49	0.33	0.79	0.44	0.78	100	92
	Venous	Capitainer^®B	0.60	0.41	1.16	0.53	1.11	95	92
	Capillary	Whatman 903	0.56	0.35	1.03	0.53	1.00	100	92
	Capillary	Capitainer^®B	0.55	0.36	0.82	0.52	0.80	100	92
	Plasma	EDTA K2	nd	nd	0.31	nd	0.30	100	92
Lyso-Gb₃ +16 Da (nM)	Venous	Whatman 903	nd	nd	0.21	nd	0.20	95	92
	Venous	Capitainer^®B	nd	nd	nd	nd	nd	80	100
	Capillary	Whatman 903	nd	nd	nd	nd	nd	100	100
	Capillary	Capitainer^®B	nd	nd	nd	nd	nd	84	100
	Plasma	EDTA K2	nd	nd	nd	nd	nd	95	100
Lyso-Gb₃ +18 Da (nM)	Venous	Whatman 903	nd	nd	nd	nd	nd	100	100
	Venous	Capitainer^®B	nd	nd	nd	nd	nd	100	100
	Capillary	Whatman 903	nd	nd	nd	nd	nd	100	100
	Capillary	Capitainer^®B	nd	nd	nd	nd	nd	100	100
	Plasma	EDTA K2	nd	nd	nd	nd	nd	100	100
Lyso-Gb₃ +34 Da (nM)	Venous	Whatman 903	0.28	0.17	0.39	0.25	0.38	90	92
	Venous	Capitainer^®B	nd	nd	0.25	nd	0.25	90	92
	Capillary	Whatman 903	0.30	0.21	0.35	0.30	0.35	85	100
	Capillary	Capitainer^®B	nd	nd	0.20	nd	0.20	95	92
	Plasma	EDTA K2	nd	nd	nd	nd	nd	100	92

nd = not detected (under the limits of detection).