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. 2023 Feb 10;11(2):405. doi: 10.3390/vaccines11020405

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Evolutionary characteristics of CVA6 causing atypical HFMD available from GenBank were conducted applying MEGA 7 and Simplot 3.5.1 software. (A) The Bootscan analysis of MH371303.1/Italy/2017 based on the CDS region. The reference strains are represented in the legend. (B) Phylogenetic analyses of CVA6 based on a part of VP1 sequence (276 bp). The bootstrap test was performed with 1000 replications. All the strains are labelled using the following format: accession number/country of origin/year of isolation. The prototype strain of EVA71 marked with red circles indicates the outgroups. The prototype strain of CVA6 is marked as red triangles. The blue squares mark the representative strains causing atypical HFMD.