FIG. 1.

Citrate and malate uptake in whole cells expressing CimHbs. (A) Uptake of [1,5-¹⁴C]citrate was measured in whole cells of E. coli strain JRG4008 carrying the empty plasmid pET302 (●) and strains JRG4008 (▾), DH5α (○), BL21(DE3) (□), TOP10 (⋄), and SF100 (▿) expressing CimHbs. (B) Malate transport activity was measured in whole cells of E. coli JRG4008 carrying the empty pET302 vector (●) or expressing CimHbs (▾). (C) Expression levels of CimHbs in the cells used for the uptake experiments shown in panel A. The immunoblot of membrane extracts of strains JRG4008 carrying plasmid pET302 (lane 1) and JRG4008 (lane 2), DH5α (lane 3), BL21(DE3) (lane 4), TOP10 (lane 5), and SF100 (lane 6) expressing CimHbs were analyzed using antibodies directed against the N-terminal His tag. Molecular mass markers are indicted on the right.