Citrate and malate uptake catalyzed by CimHbs in RSO membranes. [1,5-14C]citrate uptake was measured in RSO vesicles prepared from E. coli TOP10 expressing CimHbs (A), and l-[1,4(2,3)-14C]malate uptake was measured in RSO vesicles prepared from E. coli JRG4008 expressing CimHbs (B). The membranes were either not energized (○) or energized with Asc-PMS to generate a PMF (▿), or the PMF was dissipated with 10 μM FCCP (□).