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. 2001 Oct;183(20):5862–5869. doi: 10.1128/JB.183.20.5862-5869.2001

TABLE 2.

Kinetic parameters of CimHbs for uptake activity in RSO membrane vesicles

Substrate Km (μM) Kia (μM) Vmaxd (nmol/min · mg of protein)
Citrate 12 ± 2 37 ± 5
S-citramalate 100 ± 20 b
S-malate 1,500 ± 100 167 ± 20c
a

Inhibition constant for the inhibition of citrate uptake. 

b

No significant turnover was observed. 

c

Calculated using the inhibitor constant and the rate of uptake of l-malate at 19.8 μM. 

d

Vmax, maximal rate of transport.