Figure 3.

Hypoxic HK2 enhances Arg-II levels in podocytes through TGF-β1. Experiments were performed as described in Figure 1, and podocyte lysates were prepared and subjected to (A) Immunoblotting analysis of the protein levels of Arg-II, podocin, and TGF-β1; GAPDH serves as the loading control. The quantification of the signals is presented as graphs in (B–D). (E) Podocytes were treated with wt-HK2-CM in the absence or presence of SB431542: a TGF-β1 receptor 1 inhibitor. (F) Quantification of Arg-II signal in (E). (G) Quantification of the concentration of TGF-β1 in HK2-CM measured by ELISA. * p < 0.05 between the indicated groups. n = 3; CM: conditioned media, N: normoxia, H: hypoxia.