Figure 4.

Hypoxic HK2 enhances cytoskeleton filament derangement in podocytes through TGF-β1. Experiments were performed as described in Figure 3E and subjected to Phalloidin staining of cytoskeletal actin fibers (green) in human podocytes. Nuclei were stained with DAPI (blue). Quantification of the podocytes with disrupted actin cytoskeleton (in percentage) is presented in the graph on the right. * p < 0.05 between the indicated groups. n = 3; CM: conditioned media, N: normoxia, H: hypoxia. SB431542: TGF-β1 receptor 1 inhibitor.