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. 2023 Feb 5;24(4):3146. doi: 10.3390/ijms24043146

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Preservation of implanted cells in the disc nucleus pulposus (NP) of a rat tail nucleotomy model. (A) Immunofluorescence of rat tails in the cartilage gel-injected, hFCPCs-injected, ECM-injected, healthy, and injury-only discs at six weeks after nucleotomy for human nuclei antigen (green), DAPI (blue), and merged signals. (B) Changes in the percentage of human nuclear antigen-positive cells. Immunopositivity was counted in the disc NP with low-power fields and calculated as relative to the total number of DAPI-positive cells. Data are presented with box plots (n = 4, *** p < 0.001, ns, not significant, significant difference by one-way ANOVA). hFCPCs, human fetal cartilage-derived progenitor cells; ECM, extracellular matrix.