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Comparison of WTA Methods, Environmental Workflow. This figure compares RNR values for spiked targets in samples prepared from two different WTA methods. Sequencing libraries prepared from combining equal volumes of two normalized WTA reactions, one with and one without a DNase step, are referred to as WTA DNase +/−. Sequencing libraries prepared from combining equal volumes of normalized WTA and TNA are referred to as WTA & TNA.
