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. 2023 Feb 16;24(4):4000. doi: 10.3390/ijms24044000

Figure 1.

Figure 1

The ATP1A1 CT structural domain is required for the interaction with PEDV S1. (A) PEDV S1 interacts with endogenous ATP1A1 protein. PEDV at 0.1 MOI infected IPEC-J2 cells for 36 h. Western blot of CO-IP with mouse anti-S1 mAb and rabbit anti-ATP1A1 mAb. (B) The schematic diagram of the truncated structural domains of ATP1A1. (C) Full-length ATP1A1 interacted with S1 protein. Full-length ATP1A1, truncated A1, A2, A3 and A4 structural domains were transfected, fy, to Vero-E6 cells for 24 h and then PEDV at 0.1 MOI infected cells for 24 h. Western blot of co-immunoprecipitations from lysates with mouse anti-S1 mAb and mouse anti-FLAG mAb. (D) ATP1A1 CT domain interacted with S1 protein. Vero-E6 cells were transfected with the plasmid expressing ATP1A1-CT for 24 h and were infected with PEDV at 0.1 MOI for 24 h. Western blot of co-immunoprecipitations from lysates with mouse anti-S1 mAb and mouse anti-FLAG mAb.