Figure 8.

The host protein ATP1A1 co-localizes with the PEDV S1 protein early in PEDV infection. (A,B) ATP1A1 co-localized with the S1 protein. PEDV at 0.1 MOI infected with IPEC-J2 and Vero-E6 to the specified time. Cells were then stained with antibodies corresponding to Figure 7B. The nuclei were stained with DAPI (blue). The red arrows indicate co-localized signals (scale bar = 50 μm). (C,D) 3D Rendered Image. PEDV at 0.1 MOI infected IPEC-J2 and Vero-E6 cells for 1 h; cells were stained with the antibodies, with the antibody corresponding to Figure 7B. Staining of cell nuclei using DAPI (blue) staining solution. The panel shows a three-dimensional rendering; the red arrows indicate co-localized signals.