Table 1.
PCR primers used for this study.
| Primer | Sequence (5′-3′) |
|---|---|
| ATP1A1-F a | CGGGGTACCATGGGGAAGGGGGTTGGAC |
| ATP1A1-CT-F a | CGGGGTACCATGCTGGATGACAACTTCGCCTC |
| ATP1A1-1R a | CGGGATATCCTAGGCTTGCTGAGGAACCATGTTTTTG |
| ATP1A1-2R a | CGGGATATCCTACACTGCCCTGTTACAAAGACCTG |
| ATP1A1-3R a | CGGGATATCCTAGGCATCCCTGGGGTTCACC |
| ATP1A1-4R a | CGGGATATCCTAGACGGTGCCCAGGGG |
| ATP1A1-R a | CGGGATATCCTAGTAGTAGGTTTCCTTCTCCACCC |
| PEDV-N-F b | ATGATCTGGTGGCTGCTGTC |
| PEDV-N-R b | CTTCGAAGTGGCCCTGGATT |
| Porcine-ATP1A1-F b | CTTGAGCCGAGGCTTAACAC |
| Porcine-ATP1A1-R b | GAATGCCATAGGCCAAGAAA |
| Monkey-ATP1A1-F b | CAGCAGTGGACCTATGAGCA |
| Monkey-ATP1A1-R b | CATTCCAGGGCAGTAGGAAA |
| Porcine-GAPDH-F b | GATGCTGGTGCTGAGTATGT |
| Porcine-GAPDH-R b | GGCAGAGATGATGACCCTTT |
| Monkey-GAPDH-F b | CGAGATCCCTCCAAAATCAA |
| Monkey-GAPDH-R b | TGACGATCTTGAGGCTGTTG |
a The target genes were amplified by PCR and cloned into the KpnI (bold) and EcoRV (red) sites in the pECMV-3×FLAG-N vector. b Used for relative quantitative PCR.