TABLE 4.
Susceptibility of E. coli AB2847 transformants to colicin M and phage φ80
| Strain and treatment | Strain susceptibilitya with IPTG at (mM):
|
|||
|---|---|---|---|---|
| 0 | 0.01 | 0.1 | 1 | |
| AB2847(pMALp2) | ||||
| Colicin M | 3 | 3 | 3 | 3 |
| φ80 | 8 | 8 | 8 | 8 |
| AB2847(pMFT) | ||||
| Colicin M | 3 | 3 | 2 | 1 |
| φ80 | 8 | 8 | (4) | (3) |
| AB2847(pMFT137) | ||||
| Colicin M | 3 | 3 | 3 | 2 |
| φ80 | 8 | 8 | 8 | 6 |
| AB2847(pMFTLP) | ||||
| Colicin M | 3 | 3 | 2 | 1 |
| φ80 | 8 | 8 | (5) | (4) |
a
Samples (5 μl) containing 10-fold dilutions of bacteriophage φ80 or colicin M stock solutions were spotted onto lawns of the indicated bacteria. The results are presented as the −log of the highest dilution that resulted in a confluent lysis zone. Numbers in parentheses indicate that, although the lysis zone was confluent, it was cloudy, indicating poor propagation of the phage.