TABLE 5.
Effect of overexpressed FhuA on siderophore-dependent growth and sensitivity to colicin M and bacteriophage φ80
| Strain | Plasmid | Growtha on:
|
Sensitivityb with:
|
|||
|---|---|---|---|---|---|---|
| FC at 1 mM | Cit at 10 mM | Cit at 100 mM | φ80 | Colicin M | ||
| AB2847(pMALp2) | pACYC184 | 20 | 14 | 22 | 6 | 3 |
| pSU767 | 20 | 14 | 23 | 6 | 3 | |
| AB2847(pMFT) | pACYC184 | 6 | 6 | 6 | (3) | 1 |
| pSU767 | 20 | 13 | 17 | 5 | 2 | |
| AB2847(pMFT137) | pACYC184 | 6 | 15 | 23 | 5 | 2 |
| pSU767 | 21 | 14 | 20 | 6 | 3 | |
| AB2847(pMFTLP) | pACYC184 | 6 | 13 | 21 | 4 | 2 |
| pSU767 | 20 | 14 | 21 | 5 | 3 | |
a
Growth was measured as the diameter in millimeters of the growth zone around 6-mm filter disks which had been saturated with 10 μl of the indicated solution of ferrichrome (FC) or sodium citrate (Cit). The cells were induced with 0.1 mM IPTG.
b
Tenfold dilutions of bacteriophage φ80 or colicin M were spotted onto lawns of the indicated bacteria induced with 0.1 mM IPTG. The results are presented as the −log of the highest dilution that resulted in a confluent zone of lysis. Numbers in parentheses indicate a zone of lysis that was confluent but cloudy, indicating poor propagation of the phage.