Skip to main content
. 2023 Feb 15;24(4):3930. doi: 10.3390/ijms24043930

Figure 4.

Figure 4

T-bet binds to the TGFβRI promoter. (A) Putative T-bet binding sites in the TGFβRI promoter region are marked. For comparison, similar sites in the IFN-γ promoter region are also indicated. Regions containing similar T-bet binding modules are denoted in brackets. Arrows denote transcription start sites. (B,C) Polyclonal CD8 T cells were stimulated with anti-CD3ε and anti-CD28 mAbs for 5 days prior to lysis and analysis. Chromatin was immunoprecipitated using an anti-T-bet mAb and a control mouse IgG mAb. Primers specific for the TGFβRI promoter region containing the module with similarity to the IFN-γ promoter were used to amplify a 214 bp fragment. (B) Band intensities relative to the input are shown. (C) Primers specific to regions of the TGFβRI and IFN-γ promoters were used to measure abundance by real-time PCR following control mouse IgG or anti-T-bet chromatin immunoprecipitation. The mean fold enrichment for each promoter is noted. Error bars represent the standard deviation.