Figure 3.

Time-kill assays for plazomicin in the presence of silver acetate. E. coli TOP10(pUC57AAC2Ia) was incubated at 37 °C until the cell concentration reached the CFU/mL indicated in the figure at time zero. At this moment, the culture was divided in four aliquots. An aliquot was not supplemented with any reagent. The others were each supplemented with 8 μg/mL plazomicin, 8 μM silver acetate, or both. The cultures were then incubated at 37 °C and the CFU/mL values were determined after the indicated periods. AgAcetate, silver acetate. Assays were done in duplicate, and the values are mean ± SD of two independent experiments.