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. 2023 Jan 17;11(2):197. doi: 10.3390/vaccines11020197

Figure 2.

Figure 2

Cytokine production from splenocytes of immunized mice. Splenocytes isolated from mice mock-immunized with PBS (week 11) (A) or immunized with 25 µg HBcAg-wDIII VLP (B–D) were stimulated with wDIII or equivalent volume of culture media (unstimulated). After 24 or 48 h of stimulation, the levels of IL-2 (A,B), IL-6 (A,C), and IFN-γ (A,D) produced by splenocytes were quantitated. At least two independent experiments with technical triplicates were performed for each sample and the mean concentration (pg/mL) and SD are presented. Production of cytokines by antigen stimulation is statistically significant (compared to unstimulated samples, ** indicates p values < 0.0053 for IL-6, * indicates p values < 0.0174 for IL-2 24 h and 48 h, and for IFN-γ 48 h). The differences in cytokines levels between splenocytes of HBcAg-wDIII VLP-vaccinated and PBS-injected mice are also significant (p < 0.0112 for IL-2; p < 0.0120 for IL-6; and p < 0.0171 for IFN-γ). No significant difference in levels of cytokines was detected between unstimulated and wDIII-treated splenocytes from PBS-infected mice (p > 0.05).