Figure 4.

YTHDF1 can positively regulate FTH expression in an m⁶A-dependent manner. (A,B) Relative mRNA level and protein level were measured by qRT-PCR and Western blot of FTH in YTHDF1 knockdown H1299 and A549 cells. (C,D) The relative mRNA expression and protein level were determined by qRT-PCR and Western blot of FTH after METTL3 knockdown in H1299 and A549 cells. (E) The protein level of FTH revealed by Western blot in YTHDF1 knockdown lung cancer cells with METTL3 depression, compared with negative control lung cancer cells. ^* p < 0.05; ^** p < 0.01; ^*** p < 0.001; and ^## p < 0.01.