Figure 5.

Anlotinib attenuated kidney fibrosis via the mediation of TGF-β1 signaling via ERK and AKT pathways. (a) Venn diagram showed the overlaps between anlotinib targets and renal fibrosis-related targets. (b) Protein-protein interaction (PPI) network of common targets between anlotinib and renal fibrosis. (c) The KEGG enrichment analysis of 70 targets of common targets. (d) The GO enrichment for each section is listed. (e, f) Western blot results showed that anlotinib inhibited TGF-β1-induced increases in p-AKT and p-ERK in human proximal tubular cells. Results are presented as mean ± SEM. ^∗P < 0.05 and ^∗∗P < 0.01, n = 3.