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. 2023 Feb 18;2023:7747727. doi: 10.1155/2023/7747727

Figure 2.

Figure 2

Characterization of small extracellular vesicles derived from EMPA-treated MSCs. (a) TEM imaging showed that the small extracellular vesicles of the EMPA group and the Ctrl group had similar typical cup structure (right: scale = 5 μm, left: scale = 1 μm) (n = 2). (b–d) NTA showed that there was no significant difference in particle size and concentration of small extracellular vesicles between the two groups (n = 3). (e) Western blot of maker proteins of small extracellular vesicles from the EMPA and Ctrl groups (n = 3). (f) Fluorescence microscope analysis showed that small extracellular vesicles labeled with red fluorescent dye PKH26 were endocytosed by H9c2 cells after 12 h incubation (scale = 100 μm, 20 μm) (n = 2). Data are expressed as mean ± SEM. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001.