FIG. 4.

In vivo stability of ς³² mutant proteins at 30°C. Cells of strain C600 which harbor pFN476 expressing rpoH-Δ11aa, rpoH-Δ15aa, or wild-type rpoH were grown at 30°C, pulse-labeled with [³⁵S]methionine, and chased with unlabeled methionine. Aliquots were taken at the indicated times, followed by immunoprecipitation of ς³² and quantification of the precipitated proteins relative to the largest value. Closed circles, wild-type ς³²; closed triangles, ς³²-Δ11aa; open triangles, ς³²-Δ15aa.