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. 2023 Jan 17;11(2):231. doi: 10.3390/microorganisms11020231

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Nonmetric multidimensional scaling (NMDS) model (k = 2, stress = 0.089) based upon a Bray–Curtis dissimilarity matrix of the fecal microbial profiles before (black = fresh feces = T₀) and at the different steps of the fecal manipulation procedure (red = mixed feces = T1, green = prefiltered feces = T₂, blue = final fecal filtrate = T₃). The 3 dots of each color represent the different subsamples (A, B, C). Despite global AMOVA analysis showing a heterogeneous variance between groups regarding β diversity (p-value = 0.0008), a paired test did not reveal significant differences. HOMOVA testing yielded non-statistically significant differences.