TABLE 5.
Effect of Rho on trpE and trpD transcript levels
| Strain | Relevant genotype | Tryptophan (μM) | mRNA level (%)a
|
|
|---|---|---|---|---|
| trpE | trpD | |||
| 168 | trpC2 | 20 | 51 ± 6 | 53 ± 6 |
| 168 | trpC2 | 200 | 5.0 ± 0.3 | 4.9 ± 0.4 |
| PLBS322 | rho::neo trpC2 | 20 | 100 | 100 |
| PLBS322 | rho::neo trpC2 | 200 | 43 ± 2 | 37 ± 7 |
| CB312 | WT | 0 | 14 ± 1 | 19 ± 5 |
| CB312 | WT | 200 | 5.0 ± 1.0 | 3.4 ± 0.6 |
| PLBS321 | rho::neo | 0 | 100 | 100 |
| PLBS321 | rho::neo | 200 | 48 ± 1 | 44 ± 4 |
a
The highest level of both trpE and trpD mRNAs was present in rho mutant strains grown under tryptophan-limiting conditions and was set at 100%. The values are averages of two independent experiments ± standard deviation.