Skip to main content
. 2023 Feb 17;24(4):4037. doi: 10.3390/ijms24044037

Figure 4.

Figure 4

Induction of plant resistance by transient expression of MaCFEM85 and MsWAK16. (a) Transient expression of MaCFEM85, MsWAK16, or MaCFEM85+MsWAK16 in N. benthamiana leaves. Protein expression level of MaCFEM85 and MsWAK16. Leaf samples were harvested 12 h after infiltration and total soluble protein extracts were prepared. Proteins were separated by SDS–PAGE and analyzed via immunoblot using a GFP-specific antibody. Total protein content (showing equal loading) was measured with Coomassie staining. (b) N. benthamiana leaf lesions resulting from B. cinerea infection. Lesion sizes were measured at 2 d after inoculation in a minimum of nine leaves for each treatment group. Data presented in (b) is the mean ± standard error, and Letters indicate statistical significance between groups at p < 0.05. (c) The mortality risk of aphids feeding on N. benthamiana transiently expressing MaCFEM85, MsWAK16, or MaCFEM85+MsWAK16. (d) The average number of progeny from each aphid after feeding on tobacco transiently expressing GFP, MaCFEM85, MsWAK16, or MaCFEM85+MsWAK16 for 24, 48, and 72 h, respectively. Values are shown as the mean ± standard error (n = 20).