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. 2023 Feb 9;9(1):13. doi: 10.3390/ncrna9010013

Table 1.

Circulating miRNAs and their extraction and quantification methods.

Author MicroRNA Studied RNA Extraction Method MicroRNA Quantification Method MicroRNA Quantification Technology
Liu [20] miRNA-150 mirVana™ PARIS™ kit (Invitrogen) qRT-PCR TaqMan
Galenko [17] miRNA-133a Norgen Biotek, Inc. (Thorold, ON, Canada) qRT-PCR TaqMan
Galenko [17] miRNA-150 Norgen Biotek, Inc. qRT-PCR TaqMan
Sieweke [18] miRNA-21 miRNeasy Mini kit qRT-PCR TaqMan
Xiao [21] hsa-miRNA-4443 miRCURY™ RNA isolation kit Agilent Bioanalyzer 2100 (Small RNA Analysis Chip; Agilent Technologies, Inc., Santa Clara, CA, USA) TaqMan
Chen et al. [22] miRNA-21 TRIzol reagent kit qRT-PCR TaqMan
Geurts et al. [19] miR-20a-5p Illumina NextSeq 500 (Illumina, San Diego, CA, USA) HTG Molecular Diagnostics, Tuscon, AZ, USA ------------------
Geurts et al. [19] miR-4798-3p Illumina NextSeq 500 (Illumina, San Diego, CA, USA) HTG Molecular Diagnostics, Tuscon, AZ, USA -----------------

miRNA: MicroRNA; qRT-PCR: Reverse Transcriptase-Polymerase Chain Reaction; miR: Pre-miRs.