TABLE 2.

Properties of primers used in this study

Primer	Sequence (5′–3′) and salient features	Annealing position
TadA UP		5′ end of CU1000N tadA
TadA DWN		3′ end of CU1000N tadA
TadA-T7 DWN		3′ end of CU1000N tadA
TadA K210A-1		Overlapping K210 codon and directed toward tadA 5′
TadA K210A-2		Overlapping K210 codon and directed toward tadA 3′
TadA K210Q-1		Overlapping K210 codon and directed toward tadA 5′
TadA K210Q-2		Overlapping K210 codon and directed toward tadA 3′