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. 2023 Feb 7;24(4):3259. doi: 10.3390/ijms24043259

Table 1.

Unsuccessful attempts to measure the endothelial ICRAC.

Endothelial Cell Type Measured ICRAC Agonist Used to Reduce the [Ca2+]ER Intracellular Ca2+ Buffering Reference
HUVECs No Extracellular thapsigargin 0.1 mM EGTA, no CaCl2 [135]
HUVECs No Intracellular InsP3 or extracellular thapsigargin 0.1 mM EGTA, no CaCl2; 10 mM BAPTA no CaCl2 [34]
CPAE cells No 10 mM EGTA/BAPTA 10 mM EGTA; 10 mM EGTA or 10 mM BAPTA [33]
InsP3 + 10 mM EGTA/BAPTA
Thapsigargin
InsP3 + Thapsigargin +10 mM EGTA

Abbreviations: CPAE cells: calf pulmonary artery endothelial cells; HUVECs: human umbilical vein endothelial cells.