Table 1.
Primers involved in this study.
| Primer | Sequence (5′ to 3′) | Length (bp) | Description |
|---|---|---|---|
| AFV1F | ATAGGTTCGCTCAGCCTTTTG | 21 | For SsAFV1 detection |
| AFV1R | CAGCCCTCTACACCGCATT | 19 | |
| MV9F | TATCAGGATTCATACCGAGGCA | 22 | For SsMV9 detection |
| MV9R | CACCGACAAAGGAAAGAAGGAG | 22 | |
| MV17F | AGCAGAGTGGACCAGGCTATT | 21 | For SsMV17 detection |
| MV17R | TGTTCACCCTATCCCATCATTT | 22 | |
| OlV18F | TGTGACGGCTGAGAAGTTGAA | 21 | For SsOlV18 detection |
| OlV18R | TCCCATCCTCGTTGTCTGAAT | 21 | |
| OlV14F | GGAAGACGGCAGCAGCAAA | 19 | For SsOlV14 detection |
| OlV14R | TCGCCACTCCCAGAAAAGC | 19 | |
| PC2 | CCGAATTCCCGGGATCC | 17 | For nested PCR |
| 3AFV1L | CACTCAACGCTCACTTGCTC | 20 | For 3′ terminal-sequence cloning of SsAFV1 |
| 3AFV1S | GAACGACACCACCACAATGG | 20 | |
| 5AFV1L | GACCAGGGGATGTTCGCATA | 20 | For 5′ terminal sequence cloning of SsAFV1 |
| 5AFV1S | GCGTGTGAGTGTAATTGCGT | 20 |