Figure 6.

(A–C) Electrospun nanofiber surface morphology and immunostaining. (A) PLGA and (B) RGD/PLGA nanofiber matrix images obtained by atomic force microscopy. (C) PLGA and RGD/PLGA electrospun fiber immunofluorescence images following staining with FITC-labeled anti-M13 phage antibodies (green). (D–F) The morphologies of C2C12 myoblasts cultured on PLGA and RGD/PLGA nanofiber substrates. C2C12 myoblasts were cultured for three and seven days on (D) PLGA and (E) RGD/PLGA nanofiber matrices. Nuclei of the cells were counterstained with DAPI (blue), F-actins were stained with TRITC-labelled phalloidin (red), and RGD-M13 phages were immune-stained with FITC-labelled anti-M13 phage antibodies (green). (F) Quantification of the area and aspect ratio of the cells after three days (* p < 0.05). Reproduced with permission from Ref. [30]. Copyright © 2015, Shin et al., Springer Nature.