Table 6.
LC gradient and flow rate conditions.
| Time (min) | Module | Events | Parameters |
|---|---|---|---|
| 0 | Pumps | Pump B Conc. | 0 |
| 0 | Pumps | A/B Total Flow | 0.400 |
| 0 | Pumps | Pump C Flow | 0.100 |
| 0.3 | Pumps | Pump B Conc. | 0 |
| 3.0 | Pumps | Pump B Conc. | 25 |
| 3.0 | Pumps | A/B Total Flow | 0.400 |
| 3.0 | Pumps | Pump C Flow | 0.100 |
| 3.1 | Pumps | Pump C Flow | 0 |
| 3.2 | Pumps | Pump B Conc. | 100 |
| 3.2 | Pumps | A/B Total Flow | 0.600 |
| 3.6 | Pumps | Pump B Conc. | 100 |
| 3.7 | Pumps | Pump B Conc. | 25 |
| 4.1 | Pumps | Pump B Conc. | 25 |
| 4.4 | Pumps | Pump B Conc. | 100 |
| 4.9 | Pumps | A/B Total Flow | 0.600 |
| 4.9 | Pumps | Pump B Conc. | 100 |
| 5.0 | Pumps | Pump C Flow | 0 |
| 5.1 | Pumps | Pump B Conc. | 0 |
| 5.1 | Pumps | A/B Total Flow | 0.400 |
| 5.1 | Pumps | Pump C Flow | 0.100 |
| 7.0 | Controller | Stop |