Table 1.
The efficiency of IRESs in different cell lines.
| IRES | Cell Line | Method of Comparison | Results | Source |
|---|---|---|---|---|
| EMCV, CVB3 | HEK293, HeLa, A549, Min6 | Gaussia luciferase | The efficiency of IRESs varies according to cell type; CVB3 IRES was superior in all cell types | [9] |
| EMCV, Poliovirus, KSHV, and HCV | HEK293; U87; Huh7; 293T | GFP | Poliovirus IRES resulted in maximal expression in HEK293 | [7] |
| EMCV and HAV | Monkey kidney cells (BT7-H) | antibiotic resistance (bacterial chloramphenicol acetyltransferase) | EMCV IRES was more efficient than translation directed by the HAV IRES | [24] |
| A synthetic construct with five concatenated copies of the 9-nt Gtx IRES | mouse N2a cells | Photinus luciferase | The efficiency of the translation increases, if several copies of the 9-nt Gtx IRES are included in the construct | [25] |
| EMCV, c-myc, FGF-2, and HTLV-1 | B16.F10, TS/A, NIH-3T3, ψCRIP, 293T, and primary cultures of human melanoma cells | Immunostaining and flow cytometry | The efficiency of translation initiation depends on the type of cells induced and the presence of other genetic elements in the vector. | [26] |
| Five viral (FMDV, HCV, EMCV, PV, HRV) and eight cellular IRES elements (Rbm3, NRF, Apaf-1, BIP, VCIP, AQP-4, c-myc, CAT-1) |
murine fibroblast cell line (NIH 3T3), mouse embryonic fibroblasts (MEF), human hepatoma cell line (Huh 7) and human lung fibroblasts (MRC-5) | Firefly and Renilla luciferase | Vascular endothelial growth factor and type 1 collagen-inducible protein (VCIP) IRES induced the highest firefly luciferase expression rate in all tested cell lines | [27] |