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. 2023 Feb 7;15(2):461. doi: 10.3390/v15020461

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Kinetics of H9N2 virus replication and shedding upon experimental infections in Sonali and broiler chickens. The samples that showed value of >40 considered as negative and in the graph represent as zero (0). The graphs showed the Ct values generated at RT-qPCR targeting H9 gene in organs (trachea, lungs, intestine) and swabs (oropharyngeal and cloacal) sample. In general, virus replication progressively increased at 3 and 5 dpi whereas, progressively started to decline from 7 dpi up to 10 dpi. In most cases, viral load is higher in Sonali chicken compared with broiler. No significant differences between two viruses (LT_2 and LT_56) in each infected chicken group. Asterisk (*) indicates level of significance (p < 0.05).