Figure 5.

GEF-H1 knockdown blocked the activation of RhoA and the phosphorylation of MLC2 in NPHP1^KO mice. (A,B): The expression of RhoA activation in different mice groups. (A): Western blot, (B): Densitometry analysis; n = 6 mice/group). (C,D): The phosphorylation of MLC2(p-MCL2) in murine kidneys; p-MCL2 was upregulated in the kidneys of NPHP1^KO mice compared with that in WT mice. After GEF-H1 knockdown, this upregulation was decreased in NPHP1 ^KO mice. (C): Western blot, (D): Densitometry analysis; n = 6 mice/group). Data represent the mean ± SEM. **** p < 0.0001; ns, no significance.