Item No	Recommendation	Achieved?	Section No.
Title and abstract	1	(a) Indicate the study’s design with a commonly used term in the title or the abstract	Yes	Abstract
		(b) Provide in the abstract an informative and balanced summary of what was done and what was found	Yes	Abstract
	Introduction
Background/rationale	2	Explain the scientific background and rationale for the investigation being reported	Yes	Section 1
Objectives	3	State specific objectives, including any prespecified hypotheses	Yes	Section 1 and Section 2.6
	Methods
Study design	4	Present key elements of study design early in the paper	Yes	Section 2.1
Setting	5	Describe the setting, locations, and relevant dates, including periods of recruitment, exposure, follow-up, and data collection	Yes	Section 2.2
Participants	6	(a) Cohort study—Give the eligibility criteria, and the sources and methods of selection of participants. Describe methods of follow-up Case-control study—Give the eligibility criteria, and the sources and methods of case ascertainment and control selection. Give the rationale for the choice of cases and controls Cross-sectional study—Give the eligibility criteria, and the sources and methods of selection of participants	Yes	Section 2.2
		(b) Cohort study—For matched studies, give matching criteria and number of exposed and unexposed Case-control study—For matched studies, give matching criteria and the number of controls per case	Not applicable (NA)	NA
Variables	7	Clearly define all outcomes, exposures, predictors, potential confounders, and effect modifiers. Give diagnostic criteria, if applicable	Yes	Section 2.3
Data sources/ measurement	8	For each variable of interest, give sources of data and details of methods of assessment (measurement). Describe comparability of assessment methods if there is more than one group	Yes	Section 2.2 and Section 2.3
Bias	9	Describe any efforts to address potential sources of bias	Yes	Section 2.3 and Section 2.6
Study size	10	Explain how the study size was arrived at	Yes	Section 2.2
Quantitative variables	11	Explain how quantitative variables were handled in the analyses. If applicable, describe which groupings were chosen and why	Yes	Section 2.3
Statistical methods	12	(a) Describe all statistical methods, including those used to control for confounding	Yes	Section 2.6
		(b) Describe any methods used to examine subgroups and interactions	Yes	Section 2.6
		(c) Explain how missing data were addressed	Yes	Section 2.6
		(d) Cohort study—If applicable, explain how loss to follow-up was addressed Case-control study—If applicable, explain how matching of cases and controls was addressed Cross-sectional study—If applicable, describe analytical methods taking account of sampling strategy	Yes
		(e) Describe any sensitivity analyses	Yes	Section 2.6
	Results
Participants	13	(a) Report numbers of individuals at each stage of study—eg numbers potentially eligible, examined for eligibility, confirmed eligible, included in the study, completing follow-up, and analysed	Yes	Section 2.2 and Section 2.5
		(b) Give reasons for non-participation at each stage	Yes	Section 2.5
		(c) Consider use of a flow diagram	Yes	Section 2.1
Descriptive data	14	(a) Give characteristics of study participants (eg demographic, clinical, social) and information on exposures and potential confounders	Yes	Section 2.1 and Section 3
		(b) Indicate number of participants with missing data for each variable of interest	Yes	Section 2.6 and Section 3
		(c) Cohort study—Summarise follow-up time (eg, average and total amount)	NA
Outcome data	15	Cohort study—Report numbers of outcome events or summary measures over time	NA
		Case-control study—Report numbers in each exposure category, or summary measures of exposure	NA
		Cross-sectional study—Report numbers of outcome events or summary measures	Yes	Section 2.6 and Section 3
Main results	16	(a) Give unadjusted estimates and, if applicable, confounder-adjusted estimates and their precision (eg, 95% confidence interval). Make clear which confounders were adjusted for and why they were included	Yes	Section 2.6 and Section 3
		(b) Report category boundaries when continuous variables were categorized	Yes	Section 2.3 and Section 3
		(c) If relevant, consider translating estimates of relative risk into absolute risk for a meaningful time period	NA
Other analyses	17	Report other analyses done—eg analyses of subgroups and interactions, and sensitivity analyses	Yes	Section 2.6 and Section 3
	Discussion
Key results	18	Summarise key results with reference to study objectives	Yes	Section 4
Limitations	19	Discuss limitations of the study, taking into account sources of potential bias or imprecision. Discuss both direction and magnitude of any potential bias	Yes	Section 4.2
Interpretation	20	Give a cautious overall interpretation of results considering objectives, limitations, multiplicity of analyses, results from similar studies, and other relevant evidence	Yes	Section 4.1
Generalisability	21	Discuss the generalisability (external validity) of the study results	Yes	Section 4.2
	Other information
Funding	22	Give the source of funding and the role of the funders for the present study and, if applicable, for the original study on which the present article is based	Yes	Appendix A, Appendix B and Appendix C