Table 3.
An overview of the performance of CRISPR detection based on colorimetric signal sensing.
| Method | Target | Test Time | Sensitivity | Reference |
|---|---|---|---|---|
| RAA-Cas12a based system (CORDS) | African swine fever virus (ASFV) | 60 min | 1 fM | [46] |
| RPA-CRISPR/Cas12a system | L. maculans | 45 min | 4.7 copies per test | [48] |
| CRISPR/Cas12a-LFD | ASFV | 60 min | 20 copies per test | [49] |
| CRISPR/Cas9-mediated Lateral flow nucleic acid assay (CASLFA) | L. monocytogenes, genetically modified organisms (GMOs), ASFV | 60 min | 102 copies per test | [50] |
| Lateral flow strip combined with Cas9 | double food-borne pathogens | 3 h | 102 CFU mL−1 | [51] |
| Combines the Cas12a with universal AuNPs strand-displacement probe | SARS-CoV-2 | 20 min | 2.7 × 102 CFU mL−1 | [53] |
| RT-RPA-coupled CRISPR/Cas12a colorimetric assay | SARS-CoV-2 | 60 min | 1 copy per test | [54] |