Table 4.
An overview of functional DNA-assisted CRISPR methods for different non-nucleic acid target detection.
| Method | Target | Signal Readout | Sensitivity | Reference |
|---|---|---|---|---|
| DNAzyme walkers-triggered CRISPR assay | serum amyloid A-1 protein (SAA1) and coagulation factor V (FV) | Fluorescent | 30.00 pg mL−1 for SAA1 and 200.00 pg mL−1 for FV | [69] |
| Functional DNA Regulated CRISPR-Cas12a Sensor | ATP and Na+ | Fluorescent | 4.75 μM for ATP and 0.10 mM for Na+ | [70] |
| MDANs-Cas12a | circulating tumor cells (CTCs) | Fluorescent | 26 cells mL–1 | [72] |
| CRISPR/Cas12a | exosome | Fluorescent | 103 particles μL−1 | [73] |
| APC-Cas | Salmonella Enteritidis cells | Fluorescent | 1 CFU per test | [74] |