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. 2023 Feb 15;24(4):3943. doi: 10.3390/ijms24043943

Figure 3.

Figure 3

The endogenous protein expression levels of ADM, CRLR, RAMP2 and RAMP3 in A7r5 cells (A–D) exposed to 200 μM PA for 24 h. The effects of ADM (10 nM) on cell viability (E), inflammation (F–H) and oxidative stress (I–L) stimulated by PA. The protein expression levels of proinflammatory cytokines TNF-α (F), IL-1β (G) and IL6 (H) were measured to reflect inflammation, and the NOX2 and NOX4 protein expression levels (I,J), ROS level (K) and NADPH activity (L) were detected to reflect oxidative stress. The A7r5 cells were pretreated with 10 nM ADM for 30 min subsequently treated by PA for 24 h. n = 3–6. Each value indicates mean ± SEM. * p < 0.05 vs. control group (two-tailed unpaired t-test, (A–D)). Values with the same superscript letter are not significantly different, and the different letters indicate significant differences between the groups (p < 0.05, one-way ANOVA followed by Bonferroni post hoc analysis, (E–L)).