Table 2.
Identified peptide sequences and potential bioactivities associated with a Laminaria digitata protein hydrolysate 3 kDa permeate fraction identified using MS and in silico analysis.
| Parent Protein Name and UniProt Accession Number | Peptide Single Amino Acid Sequence | f(X–X) of Parent Protein | Peptide Ranker Value 1 | Novelty (Found in BIOPEP 2 Database) | Observed Bioactivity | Simulated Digestion Using PeptideCutter 3/Peptide Digestion Fragments | Associated Predicted Bioactivities | References |
|---|---|---|---|---|---|---|---|---|
| Neopyriopia yezoensis NCBI Taxonomy ID 2788 Photosystem II CP47 reaction centre protein UniProtKB_Q1XDG4 (PBSS_NCOYE) | IGNNPAKGGLF | f(315–326) | 0.82 | Novel | ACE-1 inhibition | IGNNPAK; GGL; F | (GGL) Antimicrobial activity; Alpha-glucosidase inhibition | [31] |
| Porphyra purpurea NCBI Taxonomy ID P51322 Photosystem II CP47 reaction centre protein UniProtKB_P51322 (PSBB_PORPU) | YIGNNPAKGGLF | f(314–326) | 0.81 | Novel | ACE-1 inhibition | Y; IGNNPAK; GGL; F | Antimicrobial; Alpha-glucosidase inhibition | [31] |
| Pseudooceanicola algae OX = 1537215 UniProt KB-A0A4185KT7_9RHOB | DAALDFGPAL | f(53–62) | 0.78 | Novel | ACE-1 inhibition | DAA; L; D; F; GPAL | Antimicrobial | [32] |
| Neopyropia yezoensis (Susabi-nori) (Pyropia yezoensis) UniProtKB-Q1XDG4 (PSBB_NEOYE) Photosystem II CP47 reaction centre protein | AFYDYIGNNPAKGGLF | f(310–325) | 0.78 | Novel | ACE-1 inhibition | A; F; Y; DY; IGNNPAK; GGL; F | Antimicrobial; Alpha-glucosidase inhibition; ACE inhibitor | [33,34] |
| Ectocarpus species CCAP UniProtKB_A0A6H5TY18 (A0A6H5J418_9PHAE) LHCP protein | SDGKIFDPL | f(57–65) | 0.74 | Novel | ACE-1 inhibition | SDGK; I; F; DP; L | Dipeptidyl peptidase IV inhibitor; ACE inhibitor | [35,36] |
| Neopyropia yezoensis Photosystem II CP47 reaction centre protein PSbB UniProtKB_Q1XDG4 | YDYIGNNPAKGGLF | f(312–325) | 0.73 | Novel | ACE-1 inhibition | Y; DY; IGNNPAK; GGL; F | Alpha glucosidase inhibition; ACE inhibitor; Antimicrobial | [31,34] |
| Ectocarpus sp. CCAP 1310/34 Uncharacterised protein UniProtKB-A0A6HSJUW7_9PHAE | QGRVPGDIGFDPL | f(151–163) | 0.67 | Novel | ACE-1 inhibition | QGR; VPGDIG; F; D; PL | Antimicrobial (QGR); ACE inhibitor; (PL) ACE inhibitor | [37,38,39] |
| Ectocarpus sp. CCAP 131-/34 Transketolase_1_domain containing protein UniProtKB_10A6H5L712_9PHAE | SMSGHPGAPM | f(37–46) | 0.65 | Novel | ACE-1 inhibition | SM; SGHPGAPM | DPP-III inhibitor (SM) | [40] |
| Ectocarpus sp. CCAP 131/34 HSP90 protein UniProtKB-A0H6H5L026_9PHAE | SEFIGFPIK | f(208–216) | 0.64 | Novel | ACE-1 inhibition | SE; F; IGF; PIK | Anti-inflammatory (IGF); Antimicrobial (PIK); Stimulating vasoactive substance release (SE) | [41,42] |
| Ectocarpus siliculosus (Brown algae) Manganese stabilising protein UniProtKB-D8LG03 | GDFGNKDGKLTF | f(161–172) | 0.64 | Novel | ACE-1 inhibition | GD; F; GNK; DGK; L; TF | ACE inhibitor (GD); Fibrinogen interaction inhibitor (GNK-part of a peptide called Arietin); ACE inhibitor (TF) | [43,44,45] |
1http://distilldeep.ucd.ie/PeptideRanker/, accessed on 10 December 2022, 2https://biochemia.uwm.edu.pl/biopep-uwm/, accessed on 10 December 2022, 3 software http://web.expasy.org/peptide_cutter/, accessed on 10 December 2022.