FIG. 2.

RssA does not regulate SprE or RpoS. Complementation studies were done in stationary-phase cells grown in LB medium. RpoS and SprE levels (corresponding bands marked with labeled arrows) were monitored by Western blot analysis. (A) RpoS levels present in wild-type (wt) AF633 and rssA1::kan NR260 strains carrying the vector plasmid (pBBR1MCS), a plasmid encoding the ychJ-rssA-sprE region (pRSSA6), or one carrying the ychJ-rssA region (pRSSA9) (as marked above the lanes). (B) SprE levels present in wild-type AF633 and rssA1::kan NR260 strains carrying either pBBR1MCS, pRSSA6, or pRSSA9. The sample order is the same as for panel A. (C) RpoS levels in wild-type (wt) AF633 and rssA1::kan NR260 strains carrying either the vector plasmid pMAL-c2 (vector) or a plasmid encoding the MBP-SprE hybrid protein pMBPSprE (SprE), respectively.