FIG. 5.

RpoS regulates SprE at the transcriptional level. The β-galactosidase levels of an rssA-sprE’-lacZ⁺ fusion were measured in wild-type strains (NR507 carries the fusion at the att site and NR509 carries the fusion at the sprE chromosomal locus) and rpoS::kan strains (NR508 carries the fusion at the att site and NR510 carries the fusion at the sprE chromosomal locus) during logarithmic (hatched bars) and stationary (black bars) phases of growth in LB medium. The location where the fusion was integrated (att site or sprE locus) and the rpoS genotype are indicated. The relative levels of β-galactosidase activity are shown as the average and SD of three measurements per sample, and they are representative of at least three independent experiments.