Effects of β2-adrenergic receptor blockade on the development and maintenance of pain-like behavior in AcGP mice. (A) Experimental time course for drug pre-treatment and the behavioral test; (B,C) Paw withdrawal threshold to mechanical stimuli of AcGP mice pre-treated with butoxamine (B) or carazolol (C); (D) Experimental time course for drug post-treatment and the behavioral test; (E,F) Paw withdrawal threshold to mechanical stimuli of AcGP mice post-treated with butoxamine (E) or carazolol (F). Data are presented as mean ± SEM. (B) n = 4–18; (C) n = 8–12; (E) n = 5–6; (F) n = 6. * p < 0.05, ** p < 0.01, *** p < 0.001 for one-way ANOVA with Tukey’s post hoc test (B,C). AcGP, acid saline-induced generalized pain; PWT, paw withdrawal threshold.