. 2023 Jan 30;16(1):215–221. doi: 10.14202/vetworld.2023.215-221

Table-3.

Supplementation of Water Spinach on the utilization of M. pigra leaf and L. leucocephala leaf on in vitro gas production.

Treatment	Gas production kinetics (mL/0.2 g DM substrate)				Gas 72 h (mL/0.2 g DM substrate)

	A	B	C	A+B
T₁	0.86a	26.5^d	0.06c	27.4^c	27.2^c
T₂	−0.07^a,b	35.1^cd	0.11^a,b	35.0^b,c	34.9b,^c
T₃	0.16^a,b	33.5^c,d	0.09^b	33.7^b,c	33.6^b,c
T₄	0.25^a,b	26.6^b,c	0.09^a,b	26.8^b	26.7^b
T₅	−1.01^b	38.9^a,b	0.11^a	37.5^a,b	37.5^a,b
T₆	−1.37^a,b	25.9^a	0.09^ab	26.9^a	27.7^a
T₇	−2.84^a,b	38.1^a	0.013^a,b	37.0^a	37.0^a
SEM	0.316	1.38	0.004	1.214	1.219
p-value	0.039	0.020	0.006	0.006	0.005

A=The gas production from the immediately soluble fraction, B=The gas production from the insoluble fraction, C=The gas production rate constant for the insoluble fraction (B), A + B=The gas potential extent of gas production, L. leucocephala=Leucaena leucocephala, M. pigra=Mimosa pigra, SEM=Standard error of the mean, T₁=Mimosa pigra leaf; T₂=L. leucocephala leaf; T₃=Mimosa pigra leaf + L. leucocephala leaf (50:50); T₄=Mimosa pigra leaf + Water Spinach 0.5%; T₅=L. leucocephala leaves + Water Spinach 0.5%; T₆=Mimosa pigra leaf + Water Spinach 1%, and T₇=L. leucocephala leaf + Water Spinach 1%. ^a,b,cSuperscripts differences in the same column show significant differences among. p < 0.05 treatment