Figure-1.

Gel electrophoresis of polymerase chain reaction products showing genomic DNA amplified for the internal transcribed spacer-1 gene. Agarose gels stained with ethidium bromide depicting various and multiple animal trypanosome species and mixed human trypanosomes. M: 100 base pair DNA ladder, where the 500 bp band stains most intensely (Thermo Fisher Scientific); lanes 1, 2, and 4: 700 bp (representing Trypanosoma congolense); lane 3: 520 bp (representing Trypanosoma brucei gambiense); lane 5: 710 bp (representing T. congolense forest); lane 6: 250 bp (representing Trypanosoma vivax).