Figure 5.

Evaluation of mPTP opening. Representative curves (A) of the calcium retention capacity (CRC) expressed as Fura-FF ratio, monitored in response to subsequent 10 μM CaCl₂ pulses (shown by the triangles), as detailed in “mPTP assay” section of Materials and Methods, in untreated mitochondria (control) or in the presence of 0.1 or 1.0 mM Pzs. (B) Quantification of the mPTP is expressed as the ratio of the number of calcium pulses required to induce the mPTP in inhibited (CRC_i) and treated (CRC_o) mitochondria with different concentrations of Pzs. Data represent the mean ± SD from three independent experiments carried out on different mitochondrial preparations. * indicates significant differences with respect to the control (p ≤ 0.05). ** indicates significant differences (p ≤ 0.05) among treatments within the same Pzs.