Table 1.

Strain, plasmid, and primers used in this work.

Material	Description		Reference
Strain
GLp	GS115: pGAP-LDH Bos taurus Δpdc1		[19]
GLpm	GLp: VHb V. stercoraria Δmpc1		This work
Plasmid
	mpc-VHb-HygR		This work
Primers	Sequence (5′ → 3′)
MPC1F	CTCAGATCGATAGAGTGCAAG	MPC1F with MPC1R: 543 bp amplicon in wild type and no amplicon in transformant. RAD9F with MPC1R: amplification occurs only if insertion occurs in the locus.	This work
MPC1R	GGAGAAGCTCCATTCGAC		This work
RAD9F	CTCTATGCCTTGAACTATGTCG		This work
qVHbF	CATCTTGCCAGCCGTTAAGAAG	Relative quantification of the heterologous hemoglobin expression.	This work
qVHbR	AACAACTCCTGACCGACGATAG		This work
qACT1F	TGTTGGTTGTCCTCGGTTGA	Constitutive control for quantitative PCR.	This work
qACT1R	TGAGCTTGGATTCGGCAGAT		This work